点击图片查看原图
Sortase A
Description
The Sortase A Assay Kit is a fluorogenic assay kit designed to measure Sortase A proteolytic activity for screening and profiling applications.
Figure 1: Illustration of the assay principle.
The fluorescence emitted by donor DNP is quenched due to the proximity of the Abz acceptor in the intact peptide. The protease the peptide between T (threonine) and G (glycine) and forms an intermediate complex between the cysteine residue within the active site and the C-terminus of the peptide substrate. The substrate is then covalently bounded to the triglycine nucleophile. The glycine-DNP fragment is released, freeing the emission of fluorescence by DNP.
更多详情：http://www.bpsbioscience.com.cn/Products-37982958.html
https://www.chem17.com/st542058/product_37982958.html
